User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/17

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Making Hydrogels Six different hydrogels were made using three different molecular weights of poly(vinyl alcohol) 130,000 MW 2g:10mL ratio; 6.010 g total for 30mL solution mixed and heated until homogeneous, put in freezer at 11:15AM 0.5g:10mL ratio; 1.4995 g total for 30mL solution mixed and heated until homogeneous, put in freezer at 2:00PM 89,000-98,000 MW 2g:10mL ratio; 5.9956 g total for 30mL solution mixed and heated until homogeneous, put in freezer at 11:40AM 0.5g:10mL ratio; 1.501 g total for 30mL solution mixed and heated until homogeneous, put in freezer at 11:30AM 31,000-50,000 MW 2g:10mL ratio; 5.9907 g total for 30mL solution mixed and heated until homogeneous, put in freezer at 12:25PM 0.5g:10mL ratio; 1.5006 g total for 30mL solution mixed and heated until homogeneous, put in freezer at 12:35PM Hydrogel Infused with Protein and Gold Four different hydrogels infused with 17 μM BSA protein and 2.5 mM gold were made using three different molecular weights of poly(vinyl alcohol) 0.5g solutions 0.5051g of 130,000 MW poly(vinyl alcohol), 8.08mL of water; 1mL of protein and 1mL of gold added after homogeneous gel was made put into the oven at 3:10PM 0.5002g of 89,000-98,000 MW poly(vinyl alcohol), 8.00mL of water; 1mL of protein and 1mL of gold added after homogeneous gel was made put into the oven at 2:00PM 0.5001g of 31,000-50,000 MW poly(vinyl alcohol), 8.00mL of water; 1mL of protein and 1mL of gold added after homogeneous gel was made put into the oven at 3:50PM 1.6g solution - 31,000-50,000 MW poly(vinyl alcohol) 1.6002g poly(vinyl alcohol), 8.00mL water; 1mL of protein and 1mL of gold added after homogeneous gel was made put into the oven at 3:30PM