Difference between revisions of "User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/03"

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(Entry title)
(Data)
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==Data==
 
==Data==
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* We ran 5 main tests and several of the tests multiple times to confirm the data. The multiple tests showed that the experiment was not really reproducible because we seemed to be getting different spectras every time.
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Trial run A'''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Cuvet||hydrogen peroxide concentration (mM)||Iodophenol in DMSO concentration (μM)||AAP Concentration (μM)||HRP concentration (μM)||μL of gold np added||μL of water added
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|-
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| 1( standard)||1.7||18||156.25||2.3||0||630
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|-
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| 1||1.7||18||156.25||2.3||20||610
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|-
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| 2||1.7||18||156.25||2.3||40||590
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|-
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| 3||1.7||18||156.25||2.3||60||570
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|-
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| 4||1.7||18||156.25||2.3||80||550
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|-
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| 5||1.7||18||156.25||2.3||100||530
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|-
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| Total volume added||750 μL||10 μL||35 μL||22 μL||||
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|}
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The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction. 
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Revision as of 20:57, 4 October 2012

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Kinetics

Goals

  • Run UV-Vis of Absorbance vs. time of the HRP assay.

Procedure

  • We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction.

Data

  • We ran 5 main tests and several of the tests multiple times to confirm the data. The multiple tests showed that the experiment was not really reproducible because we seemed to be getting different spectras every time.
Trial run A ' ' ' ' ' '
Cuvet hydrogen peroxide concentration (mM) Iodophenol in DMSO concentration (μM) AAP Concentration (μM) HRP concentration (μM) μL of gold np added μL of water added
1( standard) 1.7 18 156.25 2.3 0 630
1 1.7 18 156.25 2.3 20 610
2 1.7 18 156.25 2.3 40 590
3 1.7 18 156.25 2.3 60 570
4 1.7 18 156.25 2.3 80 550
5 1.7 18 156.25 2.3 100 530
Total volume added 750 μL 10 μL 35 μL 22 μL

The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction.