User:Moira M. Esson/Notebook/CHEM-581/2013/02/13: Difference between revisions
(fix raw html notebook nav) |
|||
(13 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 30: | Line 30: | ||
#Starting wavelength: 500nm | #Starting wavelength: 500nm | ||
#End wavelength: 650nm | #End wavelength: 650nm | ||
#Excitation wavelength: | #Excitation wavelength: 526nm | ||
#Exitation slit: 10 | #Exitation slit: 10 | ||
#Emission slit: 10 | #Emission slit: 10 | ||
Line 43: | Line 43: | ||
Spectra Obtained: | Spectra Obtained: | ||
<br> | <br> | ||
Figure 1. Diffusion test fluorescence spectra for hydrogel prepared as a 90:10 ratio of PVA MW 146,000-186,000:110% Lamponite | |||
<br> | |||
[[Image:Diffusion test 90 MW 146 LP correct.png]] | |||
<br> | |||
Figure 2. Diffusion test fluorescence spectra for hydrogel prepared as a 90:10 ratio of PVA MW 146,000-186,000:110% NaMT | |||
<br> | |||
[[Image:Rhodamine diffusion test 90 MW 146 110 NaMT.png]] | |||
<br> | |||
Figure 3. Diffusion test fluorescence spectra for hydrogel prepared as 90:10 ratio of PVA MW 130,000:Lamponite | |||
<br> | |||
[[Image:Rhodamine diffusion test 90 MW 130 LP.png]] | |||
<br> | |||
Figure 4. Diffusion test fluorescence spectra for hydrogel prepared as a 90:10 ratio of PVA MW 130,000: 50% NaMT | |||
<br> | |||
[[Image:Rhodamine Diffusion test 90 MW 130 50%NaMT.png]] | |||
<br> | |||
Figure 5. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 130,000:50% NaMT | |||
<br> | |||
[[Image:Rhodamine Diffusion test 50 PVA 50% NaMT.png]] | |||
<br> | |||
Figure 6. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 146,000: Lamponite | |||
<br> | |||
[[Image:Rhodamine Diffusion test 50 MW 146 LP.png]] | |||
<br> | |||
Figure 7. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 146,000: 110% NaMT | |||
<br> | |||
[[Image:Rhodamine diffusion test 50 MW 146 110%NaMT.png]] | |||
<br> | |||
Figure 8. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 130,000: NaMT | |||
<br> | |||
[[Image:Rhodamine diffusion test 50 MW 130 NaMT.png]] | |||
<br> | |||
Figure 9. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 146,000-186,000: 110% Lamponite | |||
<br> | |||
[[Image:Rhodamine diffusion test 50 MW 146 110%LP .png]] | |||
<br> | |||
Figure 10. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 130,000: Lamponite | |||
<br> | |||
[[Image:Rhodamine Diffusion test 50 MW 130 LP.png]] | |||
<br> | |||
==General Observations== | ==General Observations== | ||
*Further tests will need to be done concerning 110% exchanged NaMT in order to conclude the reason for a shifted fluorescence peak. Possible causes could be an exchange between Rhodamine 6G and the exchanged clay, thus causing a shift in the wavelength of maximum absorbance. Another possible explanation could be that the exchanged 110% NaMT fluoresces on its own. | *Further tests will need to be done concerning 110% exchanged NaMT in order to conclude the reason for a shifted fluorescence peak. Possible causes could be an exchange between Rhodamine 6G and the exchanged clay, thus causing a shift in the wavelength of maximum absorbance. Another possible explanation could be that the exchanged 110% NaMT fluoresces on its own. | ||
* All prepared hydrogels have a very low level of absorbance. As such, any Rhodamine 6G and fluorescence obtained from pressure tests involving these hydrogels will be solely from the sheer pressure. Diffusion of the Rhodamine 6G occurs at a slow enough rate, taking longer than 1 week, that the pressure tests conducted will measure the amount diffusion caused due to sheer pressure. | |||
Latest revision as of 22:27, 26 September 2017
Project name | Main project page Previous entry Next entry |
Objectives
Notes
FluorescenceRhodamine 6G limit of detection:
(1μM)(5000μL)/(92μM)=54.34μL
(0.5μM)(5000μL)/(92μM)=27.17μL
(0.25μM)(5000μL)/(0.5μM)=2.5mL
General information on the parameters of the fluorescence run:
Hydrogel Fluorescence:
General Protocol:
Spectra Obtained:
General Observations
|