User:Michael F. Nagle/Notebook/Chem 571/2012/11/14: Difference between revisions
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*Start dialysis with ADA solutions to remove salts | *Start dialysis with ADA solutions to remove salts | ||
*Run ADA kinetic assay | *Run ADA kinetic assay | ||
**This is so next we can compare it to an activity assay with varying concentrations of AuNPs to determine whether they interact with the active site, deactivating it. | |||
==Procedure== | ==Procedure== | ||
#Nucleation of Au with ADA | #Nucleation of Au with ADA | ||
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#ADA Kinetics | #ADA Kinetics | ||
##[[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/11/14|Puja Moody]] calculated molar absortivity of adenosine, inosine and ADA at λ265 and determined that wavelength should be monitered because shows the most significant increase relative to adenosine concentration. | ##[[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/11/14|Puja Moody]] calculated molar absortivity of adenosine, inosine and ADA at λ265 and determined that wavelength should be monitered because shows the most significant increase relative to adenosine concentration. | ||
##Adenosine and inosine were analyzed just as they were [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/ | ##Adenosine and inosine were analyzed just as they were [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/13|yesterday]] and molar absortivity was recalculated, this time resulting in values contrary to those before. It was determined from this that an increase in absorbance actually signified an increase in inosine, and a decrease in adenosine. | ||
##The samples analyzed contained 2.7mL sodium phosphate buffer at pH 7.4 and .3mL 1mM adenosine with 15µL, 25µL, 40µL, and 50µL 65µM ADA solution. | ##The samples analyzed contained 2.7mL sodium phosphate buffer at pH 7.4 and .3mL 1mM adenosine with 15µL, 25µL, 40µL, and 50µL 65µM ADA solution. | ||
Revision as of 05:40, 7 December 2012
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Objectives
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