Difference between revisions of "User:Megan L. Channell/Notebook/Horseradish/2013/09/24"

From OpenWetWare
Jump to: navigation, search
(Protocol)
Line 20: Line 20:
 
**The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
 
**The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
 
**For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.
 
**For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.
 +
 +
==Data==
  
  

Revision as of 17:19, 30 September 2013

BDLlogo notext lr.png Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Use pepsin to cleve the peptide bonds in hemoglobin and observe its catalytic activity with and without pepstatin. The data collected will be used in a future experiment.

Protocol

The procedure for the lab can be found in Dr. Harting's lab notebook.

  • Noted differences:
    • Instead of 1.7 M perchloric acid, it was 1 M of perchloric acid.
    • For the concnetration of pepstatin added, the group decided upon 0.2 μM.
    • The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
    • For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.

Data