User:Megan L. Channell/Notebook/Horseradish/2013/09/18

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Oxidation and Reduction HRP

Objective

Perform a redox titration on Horseradish Peroxidase to determine the standard potential.

Protocol

The procedure used for the lab comes from Dr. Hartings lab notebook. A total of 5 groups analyzed the cell potential. Group CMJ went first and in the middle of the five runs, the amount of sodium dithionite added to the cell was adjusted. The amounts and concentrations of sodium dithionite added were kept for each group.

Data

The class data follows below: CHEM571 09.18.13 potassiumHRP Echem REDOX table.png CHEM571 09.18.13 potassiumHRP Echem REDOX graph.png

Calculations

  • To determine % oxidized, the absorbance peak of 405.32 nm was used from the class data.
  • [(Absorbance Value for data pt- Absorbance Min)/(Absorbance Max- Absorbance Min)]*100=% oxidized
  • The % Oxidized was subtracted from 100 to calculate the % reduced.