User:Matt Hartings/Notebook/Photosynthesis/2013/02/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
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==Objective==
==Ni Hb==
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I am filtering the Ni Hb that was previously expressed and centrifuged.
Vacuum filtration into a erlenmeyer flask that had been rinsed with bleach and ethanol. The filter aparatus was also rinsed with bleach and ethanol.
 
==Buffers==
I've had lots of mold growing in my buffers lately. So, I'm going to start autoclaving ALL of them.  




Latest revision as of 22:30, 26 September 2017

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Ni Hb

I am filtering the Ni Hb that was previously expressed and centrifuged. Vacuum filtration into a erlenmeyer flask that had been rinsed with bleach and ethanol. The filter aparatus was also rinsed with bleach and ethanol.

Buffers

I've had lots of mold growing in my buffers lately. So, I'm going to start autoclaving ALL of them.



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