Difference between revisions of "User:Matt Hartings/Notebook/Photosynthesis/2013/02/28"

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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
 
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==Objective==
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==Ni Hb==
Learn how to maintain an OpenWetWare Notebook.
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I am filtering the Ni Hb that was previously expressed and centrifuged.
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Vacuum filtration into a erlenmeyer flask that had been rinsed with bleach and ethanol. The filter aparatus was also rinsed with bleach and ethanol.
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==Buffers==
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I've had lots of mold growing in my buffers lately. So, I'm going to start autoclaving ALL of them.  
  
  

Latest revision as of 22:30, 26 September 2017

Hartings AU Photosynthesis Lab Header.png Protein Re-engineering Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png



Ni Hb

I am filtering the Ni Hb that was previously expressed and centrifuged. Vacuum filtration into a erlenmeyer flask that had been rinsed with bleach and ethanol. The filter aparatus was also rinsed with bleach and ethanol.

Buffers

I've had lots of mold growing in my buffers lately. So, I'm going to start autoclaving ALL of them.