Difference between revisions of "User:Matt Hartings/Notebook/Photosynthesis/2013/01/18"

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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
 
|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Objective==
 
==Objective==
Learn how to maintain an OpenWetWare Notebook.
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Measure stock DNA solutions
  
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==Procedure==
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Dilute samples (2 uL of DNA, 198 uL of water)
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Measure absorbance.
 +
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Assume: for dsDNA, an absorbance of 1 corresponds to a concentration of 50ug/mL and for oligos an absorbance of 1 corresponds to a concentration of 20ug/mL
 +
When I could, I used data sheets from IDT for epsilon values of oligos
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==Data==
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[[Image:20130118_DNA_UVVis.png|400px]]
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Important notes:
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# I need to do new mini/midi-preps for WT sw Mb and pQE-80 as the concentrations were just so low.
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# Asc Hb T109C f 136ug/mL (from assumption)
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# Asc Hb T109C r 272ug/mL (from assumption)
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# sw Mb M131T f 60.5 mmol/mL (from data sheet)
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# sw Mb M131T r 10.3 mmol/mL (from data sheet)
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# sw Mb pQE80 clone f 95.1 mmol/mL (from data sheet)
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# sw Mb pQE80 clone r 30.4 mmol/mL (from data sheet)
  
 
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Latest revision as of 21:22, 26 September 2017

Hartings AU Photosynthesis Lab Header.png Protein Re-engineering Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png



Objective

Measure stock DNA solutions

Procedure

Dilute samples (2 uL of DNA, 198 uL of water)

Measure absorbance.

Assume: for dsDNA, an absorbance of 1 corresponds to a concentration of 50ug/mL and for oligos an absorbance of 1 corresponds to a concentration of 20ug/mL When I could, I used data sheets from IDT for epsilon values of oligos

Data

20130118 DNA UVVis.png

Important notes:

  1. I need to do new mini/midi-preps for WT sw Mb and pQE-80 as the concentrations were just so low.
  2. Asc Hb T109C f 136ug/mL (from assumption)
  3. Asc Hb T109C r 272ug/mL (from assumption)
  4. sw Mb M131T f 60.5 mmol/mL (from data sheet)
  5. sw Mb M131T r 10.3 mmol/mL (from data sheet)
  6. sw Mb pQE80 clone f 95.1 mmol/mL (from data sheet)
  7. sw Mb pQE80 clone r 30.4 mmol/mL (from data sheet)