User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/23: Difference between revisions
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==Description== | ==Description== | ||
Make a 37C water bath that can hold at least 3 test tubes (same size as you used yesterday) at a time --- START THIS FIRST | |||
# Pepsin Digest | # Pepsin Digest | ||
## Stock Solutions | ## Stock Solutions | ||
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#### Measure the absorbance at 280 --- This will be your A<sub>280</sub>(Test) | #### Measure the absorbance at 280 --- This will be your A<sub>280</sub>(Test) | ||
### Calculate the number of units/mg of active enzyme in each sample | ### Calculate the number of units/mg of active enzyme in each sample | ||
#### Units/mg = [A<sub>280</sub>(Test) - A<sub>280</sub>(Blank)]*1000/[10minutes*mg enzyme in reaction mixture] | #### Units/mg = [A<sub>280</sub>(Test) - A<sub>280</sub>(Blank)]*1000/[10minutes*mg enzyme in reaction mixture] | ||
# Protein-AuNP analysis | # Protein-AuNP analysis | ||
## Take a picture and note physical description of each ratio | ## Take a picture and note physical description of each ratio |
Revision as of 09:08, 23 October 2013
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ObjectiveTo measure pepsin activity of 1) pepsin in solution and 2) pepsin-AuNPs in solution. We're going to try a slightly different protocol for this since the previous one see here didn't give the best results. The protocol for today is based off of this. We'll also be taking spectra of the pepsin-AuNP samples you made yesterday. DescriptionMake a 37C water bath that can hold at least 3 test tubes (same size as you used yesterday) at a time --- START THIS FIRST
Data
NotesThis area is for any observations or conclusions that you would like to note.
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