User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence and FCS Data</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==June 27, 2013==
* Insert content here...
===DNA/ThT Samples for Calibration Graph===
*Remade samples at 500, 600, 700, 800, 900nM
*Fluorescence measurements taken
====Results====
[[Image:Fluor_data_DNA_ThT_compliation.png]]<br.>
<br.>
[[Image:Fluor_data_DNA_ThT_compliation_calibration.png]]<br.>
<br.>
=====Inconsistent Spectra=====
[[Image:Fluor_data_600nM_DNA_comparison.png]]<br.>
<br.>
[[Image:Fluor_data_700nM_DNA_comparison.png]]<br.>
<br.>
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
<br.>
===Notes===
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
 




Revision as of 08:41, 27 June 2013

Fluorescence and FCS Data <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

<br.> <br.> <br.> <br.>

Inconsistent Spectra

<br.> <br.> <br.> <br.> <br.> <br.>

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.




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