# Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/05"

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## March 5, 2013

##### Dilution of Molecular Beacon=
• 50000pM: (1μM)V=(500000pM)(1mL)

25μL 1μM MB in 475μL buffer

• 25000pM: (50000pM)V=(25000)(40μL)

20μL 50000pM in 20μL buffer

• 5000pM: (50000pM)V=(5000)(40μL)

4μL 50000pM in 36μL buffer

• 500pM: (5000pM)V=(500)(40μL)

4μL 5000pM in 36μL buffer

• 100pM: (5000pM)V=(100)(40μL)

1.25μL 50000pM in 38.75μL buffer

• 1nm: (50000pM)V=(1000pM)(40μL)

1.25μL 50000pM in 38.75μL buffer

##### Sample Data
• 1:1 1nM <br.>

τ1=3.5014 ms <br.> N=9.2

• 1:5 1nM <br.>

τ1=0.7290 ms <br.> N=10

• 1:10 1nM <br.>

τ1=0.3791 ms <br.> N=10.9

• 5:1 1nM <br.>

τ1=0.6540 ms <br.> N=8

• 10:1 1nM <br.>

τ1=0.4783 ms <br.> N=14

• 50:1 1nM <br.>

τ1=0.4286 ms <br.> N=3.5

• 5:1 500pM <br.>

τ1=0.7030 ms <br.> N=20

• 10:1 500pM <br.>

τ1=8.0116 ms <br.> N=7.5

• 50:1 500pM <br.>

τ1=90515.3 ms <br.> N=1.91