Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/12"

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(February 12, 2013)
(February 12, 2013)
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#2.0μM 8 strand DNA in 3.5μM ThT
 
#2.0μM 8 strand DNA in 3.5μM ThT
 
#1.5μM 8 strand DNA in 3.5μM ThT
 
#1.5μM 8 strand DNA in 3.5μM ThT
 +
#3.5μM ThT (for reference)
  
* Fluorescence spectra for varying concentrations of 4 strand DNA:
+
* Fluorescence spectra  
[[Image:4_repeat_DNA_ThT_flouresence.jpg]]
+
[[Image:4_repeat_DNA_flouresence(ThTsubtracted).jpg]] <br>
  
* Fluorescence spectra for varying concentrations of 8 strand DNA:
+
[[Image:8_repeat_DNA_flouresence(ThTsubtracted).jpg‎]]<br>
[[Image:8repeat_DNA_and_ThT_flouresence.jpg]]
 
  
 
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Revision as of 10:12, 12 February 2013

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February 12, 2013

  • Fluorescence spectra were taken of the following solutions:
  1. 3.5μM 4 strand DNA in 3.5μM ThT
  2. 3.0μM 4 strand DNA in 3.5μM ThT
  3. 2.5μM 4 strand DNA in 3.5μM ThT
  4. 2.0μM 4 strand DNA in 3.5μM ThT
  5. 1.5μM 4 strand DNA in 3.5μM ThT
  6. 3.5μM 8 strand DNA in 3.5μM ThT
  7. 3.0μM 8 strand DNA in 3.5μM ThT
  8. 2.5μM 8 strand DNA in 3.5μM ThT
  9. 2.0μM 8 strand DNA in 3.5μM ThT
  10. 1.5μM 8 strand DNA in 3.5μM ThT
  11. 3.5μM ThT (for reference)
  • Fluorescence spectra

4 repeat DNA flouresence(ThTsubtracted).jpg

8 repeat DNA flouresence(ThTsubtracted).jpg