Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/02"

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(Autocreate 2013/04/02 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
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==Purpose==
* Insert content here...
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* The purpose of the experiment today is to make starter culture in order to prepare for a 15-hour cell growth experiment tomorrow.
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==Procedure==
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* 3mL of starter culture, each in 4 falcon tubes were made.
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* In 4 falcon tubes labeled "DH5α-T1 starter culture", 3mL of autoclaved LB were pipetted, respectively.
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* With a wooden stick, a portion of E.coli cells were picked from cell plates made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/12|02/12/2013]] and dipped in one falcon tube containing 3mL of autoclaved LB.
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* The above procedure was repeated three more times.
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* Cultures were placed in shaker at 37°C, 230rpm for 2 hours.
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* After shaking, culture were placed in centrifuge at 4°C, 4700rpm, for 30 minutes.
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* Supernatants were discarded, and pellet were stored in -80°C until next day morning.
  
  

Latest revision as of 21:35, 26 September 2017

Owwnotebook icon.png Experimental Biological Chemistry II Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Purpose

  • The purpose of the experiment today is to make starter culture in order to prepare for a 15-hour cell growth experiment tomorrow.

Procedure

  • 3mL of starter culture, each in 4 falcon tubes were made.
  • In 4 falcon tubes labeled "DH5α-T1 starter culture", 3mL of autoclaved LB were pipetted, respectively.
  • With a wooden stick, a portion of E.coli cells were picked from cell plates made on 02/12/2013 and dipped in one falcon tube containing 3mL of autoclaved LB.
  • The above procedure was repeated three more times.
  • Cultures were placed in shaker at 37°C, 230rpm for 2 hours.
  • After shaking, culture were placed in centrifuge at 4°C, 4700rpm, for 30 minutes.
  • Supernatants were discarded, and pellet were stored in -80°C until next day morning.