User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/02: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/04/02 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Purpose==
* Insert content here...
* The purpose of the experiment today is to make starter culture in order to prepare for a 15-hour cell growth experiment tomorrow.
 
==Procedure==
* 3mL of starter culture, each in 4 falcon tubes were made.
* In 4 falcon tubes labeled "DH5α-T1 starter culture", 3mL of autoclaved LB were pipetted, respectively.
* With a wooden stick, a portion of E.coli cells were picked from cell plates made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/12|02/12/2013]] and dipped in one falcon tube containing 3mL of autoclaved LB.
* The above procedure was repeated three more times.
* Cultures were placed in shaker at 37°C, 230rpm for 2 hours.
* After shaking, culture were placed in centrifuge at 4°C, 4700rpm, for 30 minutes.
* Supernatants were discarded, and pellet were stored in -80°C until next day morning.




Latest revision as of 22:35, 26 September 2017

Experimental Biological Chemistry II Main project page
Previous entry      Next entry

Purpose

  • The purpose of the experiment today is to make starter culture in order to prepare for a 15-hour cell growth experiment tomorrow.

Procedure

  • 3mL of starter culture, each in 4 falcon tubes were made.
  • In 4 falcon tubes labeled "DH5α-T1 starter culture", 3mL of autoclaved LB were pipetted, respectively.
  • With a wooden stick, a portion of E.coli cells were picked from cell plates made on 02/12/2013 and dipped in one falcon tube containing 3mL of autoclaved LB.
  • The above procedure was repeated three more times.
  • Cultures were placed in shaker at 37°C, 230rpm for 2 hours.
  • After shaking, culture were placed in centrifuge at 4°C, 4700rpm, for 30 minutes.
  • Supernatants were discarded, and pellet were stored in -80°C until next day morning.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2013/04/02&oldid=1012333"