User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/06: Difference between revisions
From OpenWetWare
Keyun Wang (talk | contribs) No edit summary |
Keyun Wang (talk | contribs) |
||
(2 intermediate revisions by the same user not shown) | |||
Line 12: | Line 12: | ||
==Procedure== | ==Procedure== | ||
* Because agar plates made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/01/ | * Because agar plates made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/01/30|2013/01/30]] liquified, new plates were made. | ||
** 12.5g of LB media powder were weighted and placed in a 750mL Erlenmeyer flask. | ** 12.5g of LB media powder were weighted and placed in a 750mL Erlenmeyer flask. | ||
** 7.5g of Agar were weighted out and placed in the same 750mL Erlenmeyer flask. | ** 7.5g of Agar were weighted out and placed in the same 750mL Erlenmeyer flask. | ||
Line 30: | Line 30: | ||
** UV-vis spectrometer was set under photometric method, with wavelength set at 650nm. | ** UV-vis spectrometer was set under photometric method, with wavelength set at 650nm. | ||
** A distilled water sample placed in plastic cuvette was placed into the spectrometer to eliminate background noise. | ** A distilled water sample placed in plastic cuvette was placed into the spectrometer to eliminate background noise. | ||
** 2mL of DH10B and DH5α-T1 cells were individually placed into the spectrometer to measure absorbance. | ** 2mL of DH10B and DH5α-T1 cells were individually placed into a plastic cuvette into the spectrometer to measure absorbance. | ||
** The absorbance for each cell after 15 hours of growth is shown in table below: | ** The absorbance for each cell after 15 hours of growth is shown in table below: | ||
{| {{table}} | |||
* For future procedure on clay-incorporated silver, please refer to [[Melissa's Notebook]] | | align="center" style="background:#f0f0f0;"|'''Cells''' | ||
| align="center" style="background:#f0f0f0;"|'''Absorbance''' | |||
|- | |||
| DH10B||1.82 | |||
|- | |||
| DH5α-T1||1.8 | |||
|- | |||
| | |||
|} | |||
* For future procedure on clay-incorporated silver, please refer to [[User:Melissa Novy/Notebook/CHEM-572/2013/02/06|Melissa's Notebook]] | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 07:07, 8 February 2013
Experimental Biological Chemistry II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||
Purpose
Procedure
|