User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/28: Difference between revisions
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* There was a high peak at 80 Au/ADA, but it was due to the accidental intake of fibers instead of solutions. With the data point at 80Au/ADA excluded, it can be concluded that there is no relationship between mole ratio of Au/ADA and gold concentration in solutions. | * There was a high peak at 80 Au/ADA, but it was due to the accidental intake of fibers instead of solutions. With the data point at 80Au/ADA excluded, it can be concluded that there is no relationship between mole ratio of Au/ADA and gold concentration in solutions. | ||
==Procedure for Au/ADA Resuspension in Tris Buffer== | ==Procedure for Au/ADA Resuspension in Tris Buffer== | ||
* Au/ADA samples ranging from 60 to 110 were used to test resuspension of fibers using Tris buffer of different concentration and pH. | |||
* 100mM of Tris buffer made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04|2012/09/04]] were diluted on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05|2012/09/05]] to100uM, 10uM, and 1uM at pH 10.0 and pH 8.0 were used to resuspension. | |||
* Au/ADA samples with mole ratios ranging from 60 to 110 have about 7.5mL left in Falcon tube after Atomic Absorption spectrometer testing. 1mL of tris buffer of varying concentrations and pH were added into each sample to make up a 1:7.5 ratio of tris buffer to Au/ADA sample. | |||
* Table listing Tris buffer used and the pH for each Au/ADA samples were listed below: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Mole ratio of Au/ADA''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Tris buffer used[uM]''' | |||
| align="center" style="background:#f0f0f0;"|'''pH of Tris buffer''' | |||
|- | |||
| 60||1||10 | |||
|- | |||
| 70||10||10 | |||
|- | |||
| 80||100||10 | |||
|- | |||
| 90||1||8 | |||
|- | |||
| 100||10||8 | |||
|- | |||
| 110||100||8 | |||
|} | |||
* The samples were pipetted up and down for a couple of times for mixing, and let sit at room temperature. | |||
==Results for Au/ADA Resuspension in Tris Buffer== | ==Results for Au/ADA Resuspension in Tris Buffer== | ||
Revision as of 21:07, 30 November 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure for Running Au/ADA samples on UV-vis Spectrophotometer
60-70-80-90-100-110-120-130-140-150
Results for Running Au/ADA samples on UV-vis Spectrophotometer
[Insert Picture]
Procedure for Running Au/ADA samples on Atomic Absorption Spectrometer
5-8-10-15-20-25-30-40
Results for Running Au/ADA samples on Atomic Absorption Spectrometer
Absorbance = 0.0153 * Concentration + 0.045
Procedure for Au/ADA Resuspension in Tris Buffer
Results for Au/ADA Resuspension in Tris Buffer |