User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/28: Difference between revisions
From OpenWetWare
Keyun Wang (talk | contribs) |
(fix raw html notebook nav) |
||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 145: | Line 145: | ||
==Results for Au/ADA Resuspension in Tris Buffer== | ==Results for Au/ADA Resuspension in Tris Buffer== | ||
* All solutions were successfully resuspended after the addition of Tris buffer. The solution after resuspension appeared as purple homogenous mixture and are clear in color. A picture taken after resuspension of all resuspended Au/ADA samples is shown below: | |||
[[Image:Au ADA after resuspension.jpg|600px]] | |||
* The color for solution for 110 Au/ADA were clearer than other Au/ADA samples because the solution was lighter in color before resuspension. | |||
* The amount of time it took for each solution to resuspend was recorded in table below: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Mole ratio of Au/ADA''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Tris buffer used[uM]''' | |||
| align="center" style="background:#f0f0f0;"|'''pH of Tris buffer''' | |||
| align="center" style="background:#f0f0f0;"|'''Time for resuspension[sec]''' | |||
|- | |||
| 60||1||10||3 | |||
|- | |||
| 70||10||10||4 | |||
|- | |||
| 80||100||10||4 | |||
|- | |||
| 90||1||8||7 | |||
|- | |||
| 100||10||8||6 | |||
|- | |||
| 110||100||8||6 | |||
|} | |||
* From table above, it can be concluded that resuspension happened faster in solutions with Tris buffer pH 10.0 then solutions with Tris buffer pH 8.0. | |||
* It was observed that resuspension time needed was must shorter in Tris buffer with pH 10.0 than in Tris buffer with pH 8.0. Possible reason for this result can be due to the high pH introduced by Tris buffer at pH 10.0 is much higher than the isoelectric point in ADA protein. This allows proteins to become negatively charged faster. Ions in buffer with stronger ionic strength can dissociate in solution and break apart the nanoparticle aggregation by interacting with the net negative charge on protein. | |||
* On the other side, at pH 8.0, less ADA proteins make the transition in time in order to take on a whole negative charge. As a result, not all ions would be able to form charge-charge interaction with the proteins, and in total delaying the process for protein aggregation breakdown, slowing down the time for complete resuspension. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 22:17, 26 September 2017
Experimental Biological Chemistry I | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure for Running Au/ADA samples on UV-vis Spectrophotometer
60-70-80-90-100-110-120-130-140-150
Results for Running Au/ADA samples on UV-vis Spectrophotometer
Procedure for Running Au/ADA samples on Atomic Absorption Spectrometer
5-8-10-15-20-25-30-40
Results for Running Au/ADA samples on Atomic Absorption Spectrometer
Absorbance = 0.0153 * Concentration + 0.045
Procedure for Au/ADA Resuspension in Tris Buffer
Results for Au/ADA Resuspension in Tris Buffer
|