User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/27: Difference between revisions
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* Au/HRP were run with the above calibration, and a table listing the absorbance and calculated concentration in units of ppm is shown below: | * Au/HRP were run with the above calibration, and a table listing the absorbance and calculated concentration in units of ppm is shown below: | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Au/HRP samples''' | |||
| align="center" style="background:#f0f0f0;"|'''Absorbance(Abs.)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration[ppm]''' | |||
|- | |||
| 60||0.0827||3.8088 | |||
|- | |||
| 70||0.1042||5.2282 | |||
|- | |||
| 80||0.1102||5.6243 | |||
|- | |||
| 90||0.1428||7.7765 | |||
|- | |||
| 100||0.1584||8.8064 | |||
|- | |||
| 110||0.166||9.3081 | |||
|- | |||
| 120||0.1901||10.8992 | |||
|- | |||
| 130||0.2754||16.5305 | |||
|- | |||
| 140||0.2144||12.5034 | |||
|- | |||
| 150||0.2473||14.6754 | |||
|} | |||
* A graph was plotted with concentration of gold in Au/HRP versus Au/HRP mole ratios shown below: | |||
[[Image:Au HRP gold.png|350px]] | |||
* From the graph, it can be seen that as the mole ratio of Au to HRP in solution, the concentration of gold in solution also increases. If the data point at 130 Au/HRP with concentration 16.5305ppm is eliminated, then one can conclude that there is a positive linear relationship between the mole ratio of Au/HRP and concentration of gold in ppm in solution before nanoparticles in solutions starts to form aggregations. | |||
* Au/ADA samples ranging from mole ratio of 60 to 150 were also run on Atomic Absorption spectrometer. Each samples with the corresponding absorbance and calculated concentration in ppm are shown in table below: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Au/ADA samples''' | |||
| align="center" style="background:#f0f0f0;"|'''Absorbance(Abs.)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration[ppm]''' | |||
|- | |||
| 60||0.0417||1.1021 | |||
|- | |||
| 70||0.0571||2.1188 | |||
|- | |||
| 80||0.0605||2.3432 | |||
|- | |||
| 90||0.0761||3.3731 | |||
|- | |||
| 100||0.1041||5.2261 | |||
|- | |||
| 110||0.0828||3.8154 | |||
|- | |||
| 120||0.0854||3.9871 | |||
|- | |||
| 130||0.102||5.083 | |||
|- | |||
| 140||0.112||5.7431 | |||
|- | |||
| 150||0.116||6.0072 | |||
|} | |||
* Concentration of gold in ppm was plotted against the mole ratio of Au to ADA ranging from 60 to 150. The graph titled "Concentration of Gold in Au/ADA [ppm] versus Au/ADA Mole Ratios" is shown below: | |||
[[Image:Au ADA gold.png|350px]] | |||
* From the graph above, it can be concluded that as the mole ratio of Au to ADA increases, the concentration of gold in solution also increases. However, in the above data, there was an outlier at 100 Au/ADA with the calculated concentration 5.2261ppm. This outlier might be due to a lack of raising before sample 100 Au/ADA was injected, leaving more gold residue to be analyzed compare to before. | |||
* It can be seen that the concentration of gold in solution in Au/HRP appeared almost double as the concentration of gold in Au/ADA solutions. This result could be explained with following reasons: | |||
** The HRP is more flexible in structure and thus can lead to more HRP folding within 85°C and 4 hours of heating. The ADA might have a less flexible structure, and might be limited with the presence of more beta sheets | |||
Revision as of 12:28, 28 November 2012
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Purpose
Procedure for Dialysis
Notes for Dialysis
Procedure for making dialyzed Au/ADA samples
60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150
Procedure for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Results for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Procedure for Running AA Spectrometer on Au/ADA and Au/HRP samples
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Results for Running AA Spectrometer on Au/ADA and Au/HRP samples
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