User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/04: Difference between revisions
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==Purpose== | ==Purpose== | ||
* Grow and amplify ADA transfected BL21(DE3) E.coli cells in LB media | * Grow and amplify ADA transfected BL21(DE3) E.coli cells in LB media | ||
* Extract cells from media after ADA protein expression | * Extract cells from media after ADA protein expression | ||
Revision as of 21:25, 23 November 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||
Purpose
Procedure
Molecular weight: 238.31g/mol 0.4M × 0.004L = 0.0016mol of IPTG needed 238.31g/mol × 0.0016mol = 0.3813g of IPTG needed
Wavelength: 600nm Settings: Photometric Data display: Absorbance
20mM Tris buffer 0.5M NaCl 30mM Imidizole pH 7.5
Notes
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