User:Karmella Haynes/Notebook/BioBrick cloning/2014/02/20: Difference between revisions
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==02/19/14== | ==02/19/14== | ||
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* | * Assembly: CMV + MV9 | ||
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'''Assemblies''' | '''Assemblies''' | ||
# | # MV9-CMV: CMV/(X/S)/700 + MV9/(X dp)/4500 | ||
* Digests (Fermentas FD) | * Digests (Fermentas FD) | ||
** | ** MV9 - use 10 μL plasmid DNA, digest with 2.0 μL XbaI | ||
** CMV - use 15 μL plasmid DNA, digest with XbaI/SpeI (1.0 μL each) | |||
{| {{table}} cellspacing="3" <!-- Digest rxn. table --> | {| {{table}} cellspacing="3" <!-- Digest rxn. table --> |
Revision as of 13:06, 20 February 2014
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02/19/14
Assemblies
Oligo annealing
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