User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/19: Difference between revisions
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==03/19/13== | ==03/19/13== | ||
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* Oligo annealing for new mammalian vector | * Oligo annealing for new mammalian vector based on MV9 | ||
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* Note: made fresh 10x annealing buffer | * Note: made fresh 10x annealing buffer | ||
# | # MV9+ K-XbaI-NLS-his-stop | ||
* | * K-XbaI-NLS-his-st 1 | ||
* | * K-XbaI-NLS-his-st 2 | ||
* | * K-XbaI-NLS-his-st 3 | ||
* | * K-XbaI-NLS-his-st 4 | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Oligo annealing rxn table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Oligo annealing rxn table --> | ||
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* [99.9°C, temp. increment -2.0°C, 1 min.; 90.0°C, temp. increment -2.0°C, 1 min.] x35 | * [99.9°C, temp. increment -2.0°C, 1 min.; 90.0°C, temp. increment -2.0°C, 1 min.] x35 | ||
* 25°C, ∞ | * 25°C, ∞ | ||
'''Vector Digest''' | |||
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Revision as of 20:32, 19 March 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||
03/19/13
Oligo annealing
Thermal Cycling (try new thermal cycling approach)
Vector Digest |