User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/06

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Owwnotebook icon.pngKarmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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03/06/13

  • Colony PCR: for Rene's project



Colony PCR

  • Streaked single colonies on gridded plates yesterday
  • Used bit of streak for PCR today
Reagent Volume Expected:
1. BB 1 = size
2. BB2 = size
Hover name
15 μL/lane; 1% agarose; Ladder
Bacteria from tip ---
10 μM VF2 primer 1.0
10 μM VR primer 1.0
2x GoTaq Green 12.5
dH2O 10.5
  25.0

PCR

  • 95°C, 3 min
  • [95°C, 15 sec; 57°C 15 sec; 72 °C 60 sec] x35
  • 72°C, 3 min
  • 4°C, ∞