User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/09

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01/09/13

  • Golden Gate assembly: hPCD + BL01 + pSB1A3



Golden Gate Assembly

  • Use purified, measured PCR products from 1/05/13
  • Use gg2 (pSB1A3), gg3, gg4; not gg1 (V0120) since it has BsmBI sites

Dilutions

DNA Conc. Vol. + dH2O
gg2 pSB1A3 67.7
gg3 hPCD 22.5
gg4 BL01 55.9