Difference between revisions of "User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/09"

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(01/09/13)
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|- valign="top"
 
|- valign="top"
 
| bgcolor=#cfcfcf | DNA
 
| bgcolor=#cfcfcf | DNA
 +
| bgcolor=#cfcfcf | length
 
| bgcolor=#cfcfcf | Conc.
 
| bgcolor=#cfcfcf | Conc.
 
| bgcolor=#cfcfcf | Vol.
 
| bgcolor=#cfcfcf | Vol.
 
| bgcolor=#cfcfcf | + dH<sub>2</sub>O
 
| bgcolor=#cfcfcf | + dH<sub>2</sub>O
 
|-
 
|-
| gg2 pSB1A3 || 67.7 || ||  
+
| gg2 pSB1A3 || 2000 || 67.7 || 7.7 || 12.3
 
|-
 
|-
| gg3 hPCD || 22.5 || ||
+
| gg3 hPCD || 186 || 22.5 || 2.1 || 17.8
 
|-
 
|-
| gg4 BL01 || 55.9 || ||
+
| gg4 BL01 || 2520 || 55.9 || 11.7 || 8.3
 
|}
 
|}
  

Revision as of 18:37, 9 January 2013

Owwnotebook icon.pngKarmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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01/09/13

  • Golden Gate assembly: hPCD + BL01 + pSB1A3



Golden Gate Assembly

  • Use purified, measured PCR products from 1/05/13
  • Use gg2 (pSB1A3), gg3, gg4; not gg1 (V0120) since it has BsmBI sites

Dilutions

DNA length Conc. Vol. + dH2O
gg2 pSB1A3 2000 67.7 7.7 12.3
gg3 hPCD 186 22.5 2.1 17.8
gg4 BL01 2520 55.9 11.7 8.3