Difference between revisions of "User:Karlena L. Brown/Notebook/PVOH Research/2013/03/22"

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(Notes)
(PVOH 130K Prepared Microsphere Samples & Dye Preparations 2)
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| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
 
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
 
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
 
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
 
 
|-
 
|-
| 50:50||NaMT||0.02530||0.02530||40||165||61
+
| 50:50||NaMT||0.02530||0.02530||40
 
|-
 
|-
| 50:50||Laponite||0.02590||0.02500||40||165||61
+
| 50:50||Laponite||0.02590||0.02500||40
 
|-
 
|-
| 90:10||NaMT||0.04140||0.01110||40||92||109
+
| 90:10||NaMT||0.04140||0.01110||40||92
 
|-
 
|-
| 90:10||Laponite||0.04470||0.01100||40||92||109
+
| 90:10||Laponite||0.04470||0.01100||40
 
|-
 
|-
| 50:50||110% CEC Laponite w/ DMHXLBR||0.02550||0.02500||40||165||61
+
| 50:50||110% CEC Laponite w/ DMHXLBR||0.02550||0.02500||40
 
|-
 
|-
| 90:10||110% CEC Laponite w/ DMHXLBR||0.04230||0.01150||40||92||109
+
| 90:10||110% CEC Laponite w/ DMHXLBR||0.04230||0.01150||40
 
|-
 
|-
| 50:50||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.02540||0.02500||40||165||61
+
| 50:50||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.02540||0.02500||40
 
|-
 
|-
| 90:10||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.04010||0.01050||40||92||109
+
| 90:10||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.04010||0.01050||40
 
|}
 
|}
  

Revision as of 16:28, 8 April 2013

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OBJECTIVES

  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 130K Prepared Microsphere Samples & Dye Preparations 2

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL)
50:50 NaMT 0.02530 0.02530 40
50:50 Laponite 0.02590 0.02500 40
90:10 NaMT 0.04140 0.01110 40 92
90:10 Laponite 0.04470 0.01100 40
50:50 110% CEC Laponite w/ DMHXLBR 0.02550 0.02500 40
90:10 110% CEC Laponite w/ DMHXLBR 0.04230 0.01150 40
50:50 50% CEC NaMT w/ Bu3HdP+ 0.02540 0.02500 40
90:10 50% CEC NaMT w/ Bu3HdP+ 0.04010 0.01050 40

Notes

  • Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
  • Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres