OBJECTIVES
- Prepare more microsphere samples expanding upon new previous method developed (new modifications)
PVOH 130K Prepared Microsphere Samples & Dye Preparations 2
RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13
1μM Rhodamine 6G Dye Concentration (90:10)
M1V1 = M2V2
1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL
1μM Rhodamine 6G Dye Concentration (50:50)
M1V1 = M2V2
1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
| PVOH vs. Clay Ratio
|
Clay Selection
|
PVOH 146K Mass (g)
|
Actual Clay Mass (g)
|
H2O Added (mL)
|
Safflower Oil Added (mL)
|
| 50:50 |
NaMT |
0.02530 |
0.02530 |
20 |
35
|
| 50:50 |
Laponite |
0.02590 |
0.02500 |
20 |
35
|
| 90:10 |
NaMT |
0.04140 |
0.01110 |
20 |
35
|
| 90:10 |
Laponite |
0.04470 |
0.01100 |
20 |
35
|
| 50:50 |
110% CEC Laponite w/ DMHXLBR |
0.02550 |
0.02500 |
20 |
35
|
| 90:10 |
110% CEC Laponite w/ DMHXLBR |
0.04230 |
0.01150 |
20 |
35
|
| 50:50 |
50% CEC NaMT w/ Bu3HdP+ |
0.02540 |
0.02500 |
20 |
35
|
| 90:10 |
50% CEC NaMT w/ Bu3HdP+ |
0.04010 |
0.01050 |
20 |
35
|
Notes
- Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
# 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
# 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
- Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres
|
PVOH Research
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