Difference between revisions of "User:Karlena L. Brown/Notebook/PVOH Research/2013/03/22"

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(OBJECTIVES)
(PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4)
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* Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres
 
* Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres
  
==PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4==
 
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
 
  
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
 
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
 
 
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
 
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
 
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
 
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
 
| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
 
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
 
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
 
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
 
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
 
|-
 
| 50:50||110% CEC NaMT w/ DMHXLBR||0.05010||0.05100||40||165||61
 
|-
 
| 90:10||110% CEC NaMT w/ DMHXLBR||0.01070||0.09100||40||92||109
 
|}
 
  
 
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Revision as of 13:38, 24 March 2013

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OBJECTIVES

  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 130K Prepared Microsphere Samples & Dye Preparations 2

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 NaMT 0.02530 0.02530 40 165 61
50:50 Laponite 0.02590 0.02500 40 165 61
90:10 NaMT 0.04140 0.01110 40 92 109
90:10 Laponite 0.04470 0.01100 40 92 109
50:50 110% CEC Laponite w/ DMHXLBR 0.02550 0.02500 40 165 61
90:10 110% CEC Laponite w/ DMHXLBR 0.04230 0.01150 40 92 109
50:50 50% CEC NaMT w/ Bu3HdP+ 0.02540 0.02500 40 165 61
90:10 50% CEC NaMT w/ Bu3HdP+ 0.04010 0.01050 40 92 109

Notes

  • Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
  • Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres