Difference between revisions of "User:Jarle Pahr/Plasmids"

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|pJP-11||Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI||7867||oriV (R2)||KanR|| ||
 
|pJP-11||Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI||7867||oriV (R2)||KanR|| ||
 
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pSB-M1g

Revision as of 07:29, 21 May 2013

Plasmid Description Length (bp) Origin of Replication Selection markers Data Comment
pJP-1 Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI oriV (RK2) KanR 6IE58_.fas
pJP-2 Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI 6105 oriV (RK2) KanR 76IE57_.fas
pJP-3 Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6093 oriV (RK2) KanR
pJP-4 Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6091 oriV (RK2) KanR
pJP-5 Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-6 Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-7 Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-8 Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6118 oriV (RK2) KanR
pJP-9 Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6105 oriV (RK2) KanR
pJP-10 Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI 6095 oriV (RK2) KanR
pJP-11 Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI 7867 oriV (R2) KanR



pSB-M1g