Difference between revisions of "User:Helen L. Slucher/Notebook/CHEM-572/2014/04/01"

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(Goals)
(Notes: Kinetics)
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==Notes: Kinetics==
 
==Notes: Kinetics==
Reduction of heme group
+
* Reduction of heme group
OPD, look for diamino...change of concentration over time, rates
+
* OPD, look for diamino...change of concentration over time, rates
Change in absorbance = change in concentration
+
* Change in absorbance = change in concentration
Find molar absorptivity
+
* Find molar absorptivity
Deoxygen/oxygen bound
+
* Deoxygen/oxygen bound
Take off clear super, spin down until it doesn't show GNP activity
+
* Take off clear super, spin down until it doesn't show GNP activity
  
  

Revision as of 09:57, 1 April 2014

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Goals

  1. Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
  2. Spin down gamma method HRP pellet
  3. Remake solutions so they are more concentrated

Notes: Kinetics

  • Reduction of heme group
  • OPD, look for diamino...change of concentration over time, rates
  • Change in absorbance = change in concentration
  • Find molar absorptivity
  • Deoxygen/oxygen bound
  • Take off clear super, spin down until it doesn't show GNP activity