User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/17: Difference between revisions
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==17 May 2013== | ==17 May 2013== | ||
Lab Meeting - see [https://docs.google.com/a/alaska.edu/document/d/1WEjKBAGd4NUJLZjGYykSZDrkruUn0_0Nxk0Gc3k3cMU/edit?usp=sharing Memos - Senile Worms] | *Lab Meeting - see [https://docs.google.com/a/alaska.edu/document/d/1WEjKBAGd4NUJLZjGYykSZDrkruUn0_0Nxk0Gc3k3cMU/edit?usp=sharing Memos - Senile Worms] | ||
Chunk ID1:B21 worms to new plate | *Chunk ID1:B21 worms to new plate | ||
-plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon! | -plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon! | ||
ROS assay with Courtney and Skyler | *ROS assay with Courtney and Skyler | ||
-transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms | -transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms | ||
-washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces | -washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces | ||
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-used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye | -used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye | ||
RESULTS | RESULTS | ||
Inoculated OP50 for seeding plates | *Inoculated OP50 for seeding plates | ||
-10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013 | -10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013 | ||
-8 ml LB w/ 4µl strep. for stock plates | -8 ml LB w/ 4µl strep. for stock plates | ||
Revaluated Schedule for RNAi treatments [https://docs.google.com/a/alaska.edu/spreadsheet/ccc?key=0At5kTTSjupzTdHhuMldJbEQtMWpBSlpTSXh0aGw4TGc&usp=sharing RNAi Screen Schedule] | *Revaluated Schedule for RNAi treatments [https://docs.google.com/a/alaska.edu/spreadsheet/ccc?key=0At5kTTSjupzTdHhuMldJbEQtMWpBSlpTSXh0aGw4TGc&usp=sharing RNAi Screen Schedule] | ||
Revision as of 15:12, 17 May 2013
 Project name
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17 May 2013
-plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon!
-transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms
-washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces
-moved samples onto 96 well plate in sections F1-3 to H1-3
-used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye
RESULTS
-10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013
-8 ml LB w/ 4µl strep. for stock plates
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