User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/17: Difference between revisions
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''' Normalized Absorbance spectrum of the reduction of HRP with sodium Na<sub>2</sub>S<sub>2</sub>O<sub>4</sub>''' | |||
[[Image:NormalizedreductionHRPzem09172013.png|750px]] | |||
*Only 10uL were added because there were noted discrepancies in the group data. | |||
Revision as of 11:18, 18 September 2013
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ObjectiveThe objective of today's lab was to determine the amount of reagent necessary to fully reduce horseradish peroxidase (HRP). HRP oxidation will be done with potassium ferricyanide K3[Fe(CN)6]. K3[Fe(CN)6], which has a reduction potential of 424mV. HRP reduction will be done with Sodium Dithionite.
HRP OxidationHRP Oxidation Protocol
UV/vis SpectraFigure 1: Normalized Absorption Spectra of the Oxidation of HRP with K3[Fe(CN)6] Figure 2: Enlarged Absorption Spectra of the Oxidation of HRP with K3[Fe(CN)6]
HRP ReductionHRP Reduction Protocol
UV/visNormalized Absorbance spectrum of the reduction of HRP with sodium Na2S2O4
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