Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/19"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Remains of the Day==
 
==Remains of the Day==
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* Finished the sequence analysis of the physical TRFLP clones - more verticillium isolated
 +
* Wrote up an outline for the tvd paper - must do real time pcr and finish writing
 
* The following table shows P. syrinagae grown on R2A for two days and X. garnderi grown on TSA for 1
 
* The following table shows P. syrinagae grown on R2A for two days and X. garnderi grown on TSA for 1
  
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! width="50"|Disease caused || width="50"|Species/Strain || width="50"|OD600 ||width="50"|Colonies at 10^-8 || width="50"|Colonies at 10^-7 ||width="50"|Colonies at 10^-6 ||width="50"|Colonies at 10^-5  
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! width="50"|Disease caused || width="50"|Species/Strain || width="50"|OD600 ||width="50"|Colonies in 25 ul at 10^-8 || width="50"|Colonies in 25 ul at 10^-7 ||width="50"|Colonies in 25 ul at 10^-6 ||width="50"|Colonies in 25 ul at 10^-5 ||width="50"|CFU per mL estimates at this OD (Average)
 
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| Bacterial speck || P. syringae DC01 || 0.129|| 3|| 21|| 38|| 141  
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| Bacterial speck || P. syringae DC01 || 0.129|| 3|| 21|| 38|| 141|| 1.2x10^10, 8.4x10^9, 1.52x10^9, 5.64x10^8, (5.621x10^9)
 
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| Bacterial speck  || P. syringae DC08|| 0.122|| 17|| 91|| 166|| lots
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| Bacterial speck  || P. syringae DC08|| 0.122|| 17|| 91|| 166|| lots|| 6.8x10^10, 3.64x10^10, 6.64x10^9, (3.50x10^10)
 
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| Bacterial speck  || P. syringae DC95|| 0.126|| 2|| 8|| 12|| 44
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| Bacterial speck  || P. syringae DC95|| 0.126|| 2|| 8|| 12|| 44|| 8x10^9, 3.2x10^9, 4.8x10^8, 1.76x10^8, (2.96x10^9)
 
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| Bacterial speck || P. syringae T6D1|| 0.123 || 8|| 24|| 85|| lots
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| Bacterial speck || P. syringae T6D1|| 0.123 || 8|| 24|| 85|| lots|| 3.2x10^10, 9.6x10^9, 3.4x10^9, (1.52x10^10)
 
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| Bacterial spot  || X. gardneri 444|| 0.129|| 189|| lots|| lots|| lots
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| Bacterial spot  || X. gardneri 444|| 0.129|| 189|| lots|| lots|| lots|| 7.56x10^11
 
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| Bacterial spot || X. gardneri DC00|| 0.122|| no growth|| no growth|| no growth|| no growth
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| Bacterial spot || X. gardneri DC00|| 0.122|| no growth|| no growth|| no growth|| no growth||
 
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| Bacterial spot || X. gardneri DC03|| 0.128 || no growth|| no growth|| no growth|| no growth
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| Bacterial spot || X. gardneri DC03|| 0.128 || no growth|| no growth|| no growth|| no growth||
 
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| Bacterial spot || X. gardneri 1B5B|| 0.138 || 54|| 86|| 116|| lots
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| Bacterial spot || X. gardneri 1B5B|| 0.138 || 54|| 86|| 116|| lots|| 2.16x10^11, 3.44x10^10, 4.64x10^9, (8.5x10^10)
 
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Latest revision as of 21:39, 26 September 2017

Owwnotebook icon.png Applied Soil Microbial Ecology Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Remains of the Day

  • Finished the sequence analysis of the physical TRFLP clones - more verticillium isolated
  • Wrote up an outline for the tvd paper - must do real time pcr and finish writing
  • The following table shows P. syrinagae grown on R2A for two days and X. garnderi grown on TSA for 1


Disease caused Species/Strain OD600 Colonies in 25 ul at 10^-8 Colonies in 25 ul at 10^-7 Colonies in 25 ul at 10^-6 Colonies in 25 ul at 10^-5 CFU per mL estimates at this OD (Average)
Bacterial speck P. syringae DC01 0.129 3 21 38 141 1.2x10^10, 8.4x10^9, 1.52x10^9, 5.64x10^8, (5.621x10^9)
Bacterial speck P. syringae DC08 0.122 17 91 166 lots 6.8x10^10, 3.64x10^10, 6.64x10^9, (3.50x10^10)
Bacterial speck P. syringae DC95 0.126 2 8 12 44 8x10^9, 3.2x10^9, 4.8x10^8, 1.76x10^8, (2.96x10^9)
Bacterial speck P. syringae T6D1 0.123 8 24 85 lots 3.2x10^10, 9.6x10^9, 3.4x10^9, (1.52x10^10)
Bacterial spot X. gardneri 444 0.129 189 lots lots lots 7.56x10^11
Bacterial spot X. gardneri DC00 0.122 no growth no growth no growth no growth
Bacterial spot X. gardneri DC03 0.128 no growth no growth no growth no growth
Bacterial spot X. gardneri 1B5B 0.138 54 86 116 lots 2.16x10^11, 3.44x10^10, 4.64x10^9, (8.5x10^10)