Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/17"

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(Autocreate 2013/04/17 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
 
(Remains of the Day)
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==Remains of the Day==
 
==Remains of the Day==
* Write up your awesome plans and results here...
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* Measured OD600s of the bacterial spot and speck grown overnight in R2A and adjusted it to around 0.12 for all of them. Diluted them to 10^-5, 10^-6, 10^-7,10^-8 and plated 25 ul of each on a quadrant of an R2A plate.
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* After reading a paper about bacterial primer testing (Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies) I found website called SILVA for testing bacterial primers - http://www.arb-silva.de/search/testprime/
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* Found paper about Burkholderia specific primers: (Molecular Method To Assess the Diversity of Burkholderia Species in Environmental Samples)
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* Found paper about Pseudomonas specific primers: (An rpoD-based PCR procedure for the identification of Pseudomonas species and for their detection in environmental samples.)
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* George showed me a great TRFLP assignment website for the program called PAT: https://secure.limnology.wisc.edu/trflp/index.jsp
  
  

Revision as of 15:11, 17 April 2013

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Remains of the Day

  • Measured OD600s of the bacterial spot and speck grown overnight in R2A and adjusted it to around 0.12 for all of them. Diluted them to 10^-5, 10^-6, 10^-7,10^-8 and plated 25 ul of each on a quadrant of an R2A plate.
  • After reading a paper about bacterial primer testing (Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies) I found website called SILVA for testing bacterial primers - http://www.arb-silva.de/search/testprime/
  • Found paper about Burkholderia specific primers: (Molecular Method To Assess the Diversity of Burkholderia Species in Environmental Samples)
  • Found paper about Pseudomonas specific primers: (An rpoD-based PCR procedure for the identification of Pseudomonas species and for their detection in environmental samples.)
  • George showed me a great TRFLP assignment website for the program called PAT: https://secure.limnology.wisc.edu/trflp/index.jsp