Difference between revisions of "User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/22"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Data==
 
==Data==
 
[[Image:Alpha Hemoglobin Pellet.png+DML|500px]]
 
[[Image:Alpha Hemoglobin Pellet.png+DML|500px]]
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The results for the hemoglobin pellet after correction
 
The results for the hemoglobin pellet after correction
  
  
 
[[Image:Alpha Myoglobin Pellet.png+DML|500px]]
 
[[Image:Alpha Myoglobin Pellet.png+DML|500px]]
 +
 
The results for the myoglobin pellet after correction
 
The results for the myoglobin pellet after correction
  
  
 
[[Image:AuNPDTT.png|500px]]
 
[[Image:AuNPDTT.png|500px]]
 +
 
AuNP solution run with Sodium Dithionite to correct protein solutions
 
AuNP solution run with Sodium Dithionite to correct protein solutions
  

Latest revision as of 22:55, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Objective

1. Testing Pellets and Supernatants of concentrated solutions

Notes Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm The pellet was much larger than before. the supernatant was removed and store for testing

Procedure

  1. The solutions were placed in the centrifuge for a second spin down for 3 hours at 1,8000 rpm
  2. Tris buffer and Sodium DIthionite in Tris buffer were degassed
  3. Ocean Optics spectroscopy of pellets and supernatants (1st and 2nd)

Data

Alpha Hemoglobin Pellet.png+DML

The results for the hemoglobin pellet after correction


Alpha Myoglobin Pellet.png+DML

The results for the myoglobin pellet after correction


AuNPDTT.png

AuNP solution run with Sodium Dithionite to correct protein solutions