Difference between revisions of "User:Bohao Liu/Notebook/Research/2014/04/30"

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(HOG Experiments : wild - type controls)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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====Settings====
 
====Settings====
 
*30 second half-period
 
*30 second half-period
*5 second imaging = 6 images per cycle
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*6 second imaging = 6 images per cycle
 
*5 minute total time = 5 complete periods
 
*5 minute total time = 5 complete periods
 +
*30 minutes in between for a total of 5 sets
 +
 +
*1 hour rest after this set of 5
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 +
*30 second half-period
 +
*6 second imaging = 6 images per cycle
 +
*20 minute total time = 20 complete periods
  
 
*ND=2
 
*ND=2
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*GFP = 200 mS exposure
 
*GFP = 200 mS exposure
 
*DIC
 
*DIC
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====Image Analysisp====
 
====Image Analysisp====
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**<code>for /l %x in (1,1,5) do MD well%x</code>
 
**<code>for /l %x in (1,1,5) do MD well%x</code>
 
**<code>for /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x</code>
 
**<code>for /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x</code>
 
  
  

Latest revision as of 23:56, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

HOG Experiments : wild - type controls and Image Analysis Parameters

Objective

  • Make sure I can do HOG experiments again
  • Confirm Wild-type values and also expand the frequencies tested
  • Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
  • See if the cells are growing

Experiment

  • Cells OD @ 10 = .453
  • Started flow at 10:45 am
  • Started experiment at 11:15 am

Settings

  • 30 second half-period
  • 6 second imaging = 6 images per cycle
  • 5 minute total time = 5 complete periods
  • 30 minutes in between for a total of 5 sets
  • 1 hour rest after this set of 5
  • 30 second half-period
  • 6 second imaging = 6 images per cycle
  • 20 minute total time = 20 complete periods
  • ND=2
  • mCherry = 200 mS exposure
  • GFP = 200 mS exposure
  • DIC


Image Analysisp

  • for windows sorting :
    • for /l %x in (1,1,5) do MD well%x
    • for /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x