User:Bohao Liu/Notebook/Research/2014/04/30

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HOG Experiments : wild - type controls and Image Analysis Parameters Objective Make sure I can do HOG experiments again Confirm Wild-type values and also expand the frequencies tested Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal See if the cells are growing Experiment Cells OD @ 10 = .453 Started flow at 10:45 am Started experiment at 11:15 am Settings 30 second half-period 6 second imaging = 6 images per cycle 5 minute total time = 5 complete periods 30 minutes in between for a total of 5 sets 1 hour rest after this set of 5 30 second half-period 6 second imaging = 6 images per cycle 20 minute total time = 20 complete periods ND=2 mCherry = 200 mS exposure GFP = 200 mS exposure DIC Image Analysisp for windows sorting : for /l %x in (1,1,5) do MD well%x for /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x