User:Bohao Liu/Notebook/Research/2014/04/29

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HOG Experiments : wild - type controls

Objective

  • Make sure I can do HOG experiments again
  • Confirm Wild-type values and also expand the frequencies tested
  • Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
  • See if the cells are growing

Experiment

  • Cells OD @ 10:30 = .389
  • Started flow at 11 am
  • Started experiment at 11:30 am

Settings

  • 240 second half-period
  • 48 second imaging = 6 images per cycle
  • 40 minute total time = 5 complete periods
  • ND=2
  • mCherry = 200 mS exposure
  • GFP = 200 mS exposure
  • DIC


Cells

  • 1:200 from 1.5 OD is at .973 OD 24 hours later
  • 1:100 set back 16 hours is at ~ .400 OD