Difference between revisions of "User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/18"

From OpenWetWare
Jump to: navigation, search
(18/12/2012)
(18/12/2012)
Line 33: Line 33:
  
 
{| border="1"
 
{| border="1"
! Header text !! Header text
+
! Dilution !! RFP (AU)
 
|-
 
|-
 
|format modifier (not displayed) | 100% ||43125
 
|format modifier (not displayed) | 100% ||43125

Revision as of 14:11, 18 December 2012

Owwnotebook icon.pngPcTF Subcloning in E. coli <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

18/12/2012

RFP Plate Reader Serial Dilution

  1. Transfer 100 μl of RFP from stock to 100μl DI water. (50% solution)
  2. Transfer 100 μl of 50% solution to 100 μl DI water. (25% solution)
  3. Transfer 100 μl of 25% solution to 100 μl DI water. (12.5% solution)
  4. Transfer 100 μl of 12.5% solution to 100 μl DI water. (6.25% solution)

Plate Reader

  • Transfer 50 μl of each solution to the Costar 96 clear bottom black side.
  • Here is the software setting:
    • Fluorescence
    • Endpoint
    • Full Plate
    • Excitation: 540, Emission: 600
    • Optics: Top
    • Gain: AutoScale
    • Light Source: Xenon Flash, Lamp Energy: High
    • Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 10
    • Read Height: 9.5 mm
  • Click OK and then Run the experiment.

Result


Dilution RFP (AU)
100% 43125
50% 16143
25% 7978
12.5% 4124
6.25% 2718