Difference between revisions of "User:Anthony Salvagno/Notebook/Research/2011/04/13/pALS PCR Troubleshooting"

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(PCR Setup)
(Results)
 
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==Results==
 
==Results==
 
+
That worked... yay! Let's move on to digestion and ligation.
 
[[Category:Ant PCR]]
 
[[Category:Ant PCR]]

Latest revision as of 08:44, 14 April 2011

Previous

The temperature in the lab has forced me to lower the temperature on the thermal cycler to 50C for annealing and that produced a PCR product. It also gave me less smear than I typically receive when doing this experiment so that is positive. I will now try to do PCR with labeled reverse primer and unlabeled forward primer and work my way through the tethering construct construction.

PCR Setup

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Results

That worked... yay! Let's move on to digestion and ligation.