Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/12/17"

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(Autocreate 2013/12/17 Entry for User:Allison_K._Alix/Notebook/Thesis_Research)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> December 17th, 2013</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==Entry title==
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1) Prepare 100mM DTT in 4% TEA
* Insert content here...
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From 5/29:
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Original DTT concentration 1M
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1M(x) = (100x10<sup>-3</sup>M)(1mL)
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x = 0.1mL = 100μL in 900μL 4% TEA
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4%(900μL) = 36μL TEA in 864μL PBS
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OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS
  
  

Revision as of 07:51, 17 December 2013

Owwnotebook icon.png December 17th, 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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1) Prepare 100mM DTT in 4% TEA

From 5/29:

Original DTT concentration 1M

1M(x) = (100x10-3M)(1mL)

x = 0.1mL = 100μL in 900μL 4% TEA

4%(900μL) = 36μL TEA in 864μL PBS

OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS