User:Allison K. Alix/Notebook/Thesis Research/2013/12/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> December 17th, 2013</span> | ||
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== | 1) Prepare 100mM DTT in 4% TEA | ||
From 5/29: | |||
Original DTT concentration 1M | |||
1M(x) = (100x10<sup>-3</sup>M)(1mL) | |||
x = 0.1mL = 100μL in 900μL 4% TEA | |||
4%(900μL) = 36μL TEA in 864μL PBS | |||
OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS | |||
Revision as of 08:51, 17 December 2013
 December 17th, 2013
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1) Prepare 100mM DTT in 4% TEA From 5/29: Original DTT concentration 1M 1M(x) = (100x10-3M)(1mL) x = 0.1mL = 100μL in 900μL 4% TEA 4%(900μL) = 36μL TEA in 864μL PBS OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS
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