Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/07/25"

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(ThT/DNA attachment)
(ThT/DNA attachment)
Line 36: Line 36:
 
4-repeat
 
4-repeat
  
1uM(1mL)=(265.6uM)x
+
2uM(500uL)=(265.6uM)x
  
x = 3.77uL in 996.23uL
+
x = 3.76uL in 496.24
  
900nM--> 90uL 1uM in 10uL PBS
+
1800nM--> 90uL 10uL PBS
  
700nM--> 70uL in 30uL
+
1400nM--> 70uL in 30uL
  
500nM--> 50uL in 50uL
+
1000nM--> 50uL in 50uL
 +
 
 +
60000nM--> 30uL in 70uL
 +
 
 +
200nM--> 10uL in 90uL
 +
 
 +
8-repeat
 +
 
 +
142.8uM(10uL) = (x)(500uL)
 +
 
 +
x= 2.856
 +
 
 +
1800nM-->  63uL in 37uL PBS
 +
 
 +
1400nM--> 49uL in 51uL
 +
 
 +
1000nM--> 35uL in 65uL
 +
 
 +
600nM--> 21uL in 79uL
 +
 
 +
200nM--> 7uL in 93uL
  
300nM--> 30uL in 70uL
 
  
100nM--> 10uL in 90uL
 
  
  

Revision as of 07:31, 25 July 2013

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To Do

  • Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
  • Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

ThT/DNA attachment

1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 100nM

b) 300nM

c) 500nM

d) 700nM

e) 900nM

      • Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively***

4-repeat

2uM(500uL)=(265.6uM)x

x = 3.76uL in 496.24

1800nM--> 90uL 10uL PBS

1400nM--> 70uL in 30uL

1000nM--> 50uL in 50uL

60000nM--> 30uL in 70uL

200nM--> 10uL in 90uL

8-repeat

142.8uM(10uL) = (x)(500uL)

x= 2.856

1800nM--> 63uL in 37uL PBS

1400nM--> 49uL in 51uL

1000nM--> 35uL in 65uL

600nM--> 21uL in 79uL

200nM--> 7uL in 93uL