User:Allison K. Alix/Notebook/Thesis Research/2013/07/25: Difference between revisions

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4-repeat
4-repeat


1uM(1mL)=(265.6uM)x
2uM(500uL)=(265.6uM)x


x = 3.77uL in 996.23uL
x = 3.76uL in 496.24


900nM--> 90uL 1uM in 10uL PBS
1800nM--> 90uL 10uL PBS


700nM--> 70uL in 30uL
1400nM--> 70uL in 30uL


500nM--> 50uL in 50uL
1000nM--> 50uL in 50uL
 
60000nM--> 30uL in 70uL
 
200nM--> 10uL in 90uL
 
8-repeat
 
142.8uM(10uL) = (x)(500uL)
 
x= 2.856
 
1800nM-->  63uL in 37uL PBS
 
1400nM--> 49uL in 51uL
 
1000nM--> 35uL in 65uL
 
600nM--> 21uL in 79uL
 
200nM--> 7uL in 93uL


300nM--> 30uL in 70uL


100nM--> 10uL in 90uL




Revision as of 08:31, 25 July 2013

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To Do

  • Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
  • Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

ThT/DNA attachment

1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 100nM

b) 300nM

c) 500nM

d) 700nM

e) 900nM

      • Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively***

4-repeat

2uM(500uL)=(265.6uM)x

x = 3.76uL in 496.24

1800nM--> 90uL 10uL PBS

1400nM--> 70uL in 30uL

1000nM--> 50uL in 50uL

60000nM--> 30uL in 70uL

200nM--> 10uL in 90uL

8-repeat

142.8uM(10uL) = (x)(500uL)

x= 2.856

1800nM--> 63uL in 37uL PBS

1400nM--> 49uL in 51uL

1000nM--> 35uL in 65uL

600nM--> 21uL in 79uL

200nM--> 7uL in 93uL






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