Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/07/25"

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(Silica-Coating Part 1)
(ThT/DNA attachment)
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==ThT/DNA attachment==
 
==ThT/DNA attachment==
  
1) Prepare the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)
+
1) Prepare 200uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)
  
a) 200nM
+
a) 100nM
  
b) 400nM
+
b) 300nM
  
c) 600nM
+
c) 500nM
  
d) 800nM
+
d) 700nM
  
e) 1uM
+
e) 900uM
  
  

Revision as of 06:52, 25 July 2013

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To Do

  • Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
  • Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

ThT/DNA attachment

1) Prepare 200uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 100nM

b) 300nM

c) 500nM

d) 700nM

e) 900uM