Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/01/15"

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(Re-running Fluorescence Measurements)
(Procedures)
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==Procedures==
 
==Procedures==
  
* Fluorescence Spectra for the following samples were obtained (excited at 515nm)
+
Obtain fluorescence spectra for the following samples:(excited at 515nm)
  
* concentrated AuNP - (3x10^-9 M)
+
* concentrated AuNP (3x10^-9 M)
 
* 1:6 diluted AuNP (6.49 x10^-9 M)  
 
* 1:6 diluted AuNP (6.49 x10^-9 M)  
* 1:6 dilution with thiol primer attached. (10μL diluted to 100μL with 90μL water)
+
* 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water)
* Elaine's Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\
+
* Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\
 
* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)
 
* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)
 +
 +
Telomerase Molecular Beacon was diluted with 1mL filtered H<sub>2</sub>O, separated into vials in 10μL aliquots and placed in the freezer.
  
 
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Revision as of 12:59, 15 January 2013

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Procedures

Obtain fluorescence spectra for the following samples:(excited at 515nm)

  • concentrated AuNP (3x10^-9 M)
  • 1:6 diluted AuNP (6.49 x10^-9 M)
  • 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water)
  • Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H2O)\
  • Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H2O)

Telomerase Molecular Beacon was diluted with 1mL filtered H2O, separated into vials in 10μL aliquots and placed in the freezer.