User:Allison K. Alix/Notebook/CHEM-581/2013/02/06
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective-Begin PVA microsphere preparation ProcedurePreparation of 20mM phosphate buffer, pH = 7.4 [Base] = monosodium phosphate, 119.98 g/mol [Acid] = disodium phosphate, 141.96 g/mol pH = pKa + log ([Base]/[Acid]) 7.4 = 7.3 + log ([Base]/[Acid]) ([Base]/[Acid]) = 1.2589 [Acid] + [Base] = 20mM [Base] = 20mM - [Acid] (20mM - [Acid])/[Acid] = 1.2589 [disodium phosphate] = 8.85mM [monosodium phosphate] = 11.14mM 8.85mmol x 141.96g/mol x 1mol/1000mmol = 1.256g disodium phosphate 11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate Calculations
Referenceshttp://www.sciencedirect.com/science/article/pii/S0168365998000893
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