User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/09/24: Difference between revisions
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==Objective== | ==Objective== | ||
*Complete the procedure as outlined by Dr.Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24|here]] | *Complete the procedure as outlined by Dr.Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24|here]].<br.> | ||
*Observe activity of pepsin and pepstatin in samples of hemoglobin. <br.> | |||
*Observe activity using UV-Vis after certain intervals of time.<br.> | |||
*At each measured interval take a sample of solution and stop the reaction to observe using SDS-PAGE. <br.> | |||
==Procedure== | ==Procedure== | ||
*Note that the hemoglobin had a concentration of 2nm in the pepsin solution.<br.> | |||
*Note that the hemoglobin had a concentration of 20nm in the pepstatin solution. <br.> | |||
==Data== | ==Data== | ||
*This graph represents the absorbance spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval. | |||
*It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin. | |||
[[Image:Sept_24_Pep's.png]] | |||
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*Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard. | |||
__NOTOC__ | __NOTOC__ |
Revision as of 12:21, 30 September 2013
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Objective
Procedure
Data
|
- Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard.