Difference between revisions of "Turner:Solutions/GAMBITMgRxn"

From OpenWetWare
Jump to: navigation, search
(New page: ==GAMBIT 2x Reaction Buffer + Mg - 10 mL== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Component''' | align="center" style="background:#f0f0f0;"|'''Final []''' | align="ce...)
 
 
Line 21: Line 21:
 
|  
 
|  
 
|-
 
|-
| pH to||7.9||with||x||(approx)||x||mL
+
| pH to||7.9||with||_||(approx)||_||mL
 
|-
 
|-
 
| sterilize by||f.s.||||||||||
 
| sterilize by||f.s.||||||||||

Latest revision as of 06:02, 31 October 2012

GAMBIT 2x Reaction Buffer + Mg - 10 mL

Component Final [] Stock [] Units mL stock ' '
Glycerol 20 50 % 4
HEPES pH 7.9 0.05 1 M 0.5
DTT 0.004 0.1 M 0.4
NaCl 0.2 5 M 0.4
MgCl2 0.01 1 M 0.1
pH to 7.9 with _ (approx) _ mL
sterilize by f.s.
store at 4C
Note: use 100x BSA from NEB as 40x stock for GAMBIT reaction