Turner:Solutions/GAMBITMgRxn: Difference between revisions
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(New page: ==GAMBIT 2x Reaction Buffer + Mg - 10 mL== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Component''' | align="center" style="background:#f0f0f0;"|'''Final []''' | align="ce...) |
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| pH to||7.9||with|| | | pH to||7.9||with||_||(approx)||_||mL | ||
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| sterilize by||f.s.|||||||||| | | sterilize by||f.s.|||||||||| | ||
Latest revision as of 07:02, 31 October 2012
GAMBIT 2x Reaction Buffer + Mg - 10 mL
| Component | Final [] | Stock [] | Units | mL stock | ' | ' |
| Glycerol | 20 | 50 | % | 4 | ||
| HEPES pH 7.9 | 0.05 | 1 | M | 0.5 | ||
| DTT | 0.004 | 0.1 | M | 0.4 | ||
| NaCl | 0.2 | 5 | M | 0.4 | ||
| MgCl2 | 0.01 | 1 | M | 0.1 | ||
| pH to | 7.9 | with | _ | (approx) | _ | mL |
| sterilize by | f.s. | |||||
| store at | 4C | |||||
| Note: use 100x BSA from NEB as 40x stock for GAMBIT reaction |
