Difference between revisions of "Talk:20.109(S13):Induce protein and evaluate DNA (Day5)"
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|Blue | |Blue |
Revision as of 14:16, 2 April 2013
Contents
Induced pellet example
TR O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD D24H/E67K/T79P/M124S | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Example | 0.95 | 1.2 (D24H) | 0.82 (G20P) | Y, bright ylw-grn |
Red | ||||
Orange | ||||
Yellow | ||||
Green | 0.34 | 1.59 (T79P) | 1.16 (M71R) | Bright Yellow |
Blue | 0.035 | 0.036 (M124S) | 0.088 (M71S) | Y, bright ylw |
Pink | ||||
Purple | ||||
Platinum |
Gels
File:S13 M2D5 TR gel-Pink.jpg
T/R Pink
WF O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD D24H/E67K/T79P/M124S | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Example | 0.95 | 1.2 (D24H) | 0.82 (G20P) | Y, bright ylw-grn |
Red | ||||
Orange | ||||
Yellow | ||||
Green | ||||
Blue | ||||
Pink | ||||
Purple | ||||
Platinum |
Gels
File:S13 M2D5 WF gel-Red.jpg
W/F Red