Difference between revisions of "Talk:20.109(F13)"

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(T/R Section)
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==COLD-PCR product gels==
 
  
===T/R Section===
 
[[Image:TR_Gel1.BMP]]
 
 
Lanes are loaded the same for each team HCC-827 exon 19, HCC-827 exon 21, SKOV3 exon 19, SKOV3 exon 21:
 
*Top gel #1:
 
*Lane 1: [https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder]
 
*Lane 2-5: White
 
*Lane 6-9: Blue
 
*Lane 10-13: Red
 
*Lane 14: Aneesh
 
 
*Bottom gel #1:
 
*Lane 1: [https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder]
 
*Lane 2-5: Orange
 
*Lane 6-9: Pink
 
*Lane 10-13: Yellow
 
*Lane 14: Aneesh
 
 
[[Image:TR_Gel2.BMP]]
 
*Top gel #2:
 
*Lane 1: https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder]
 
*Lane 2-5: Green
 
*Lane 6-9: Purple
 
*Lane 10-13: Platinum
 
 
Note: The T/R gel was completed before PCR purification.
 
 
==W/F section==
 
[[Image: WF_postpurification_gel.BMP]]
 
Lanes are loaded the same for each team HCC-827 exon 19, SKOV3 exon 19, SKOV3 exon 21:
 
*Top Gel:
 
*Lane 1: [https://www.neb.com/products/n3233-low-molecular-weight-dna-ladder Low Molecular Weight ladder]
 
*Lane 2-4: White
 
*Lane 5-7: Blue
 
*Lane 8-10: Red
 
*Lane 11-13: Orange
 
 
*Bottom Gel:
 
*Lane 1: [https://www.neb.com/products/n3233-low-molecular-weight-dna-ladder Low Molecular Weight ladder]
 
*Lane 2-4: Pink
 
*Lane 5-7: Yellow
 
*Lane 8-10: Green
 
*Lane 11-13: Purple
 
 
Note: The W/F gel was completed after PCR purification.
 

Revision as of 10:55, 16 October 2013