Difference between revisions of "Smolke:Enzyme inventory"

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(Restriction Enzymes in Stocks)
Line 13: Line 13:
 
! Enzyme !! Regular !! HF !! Regular Backup !! HF Backup
 
! Enzyme !! Regular !! HF !! Regular Backup !! HF Backup
 
|-
 
|-
! AgeI || ||Y|| ||Y  
+
! AflII ||Y|| || ||
 +
|-
 +
! AgeI ||Y||Y|| ||Y  
 
|-
 
|-
 
! AleI ||Y|| ||Y||
 
! AleI ||Y|| ||Y||
 
|-
 
|-
! ApaI ||Y|| ||Y||  
+
! ApaI ||Y|| ||YY||  
 
|-
 
|-
 
! AscI ||Y|| || ||
 
! AscI ||Y|| || ||
 
|-
 
|-
! AsiSI ||Y|| || ||
+
! AsiSI ||Y|| ||Y||
 +
|-
 +
! AvrII ||Y|| ||YY||
 
|-
 
|-
 
! BamHI ||Y||Y|| ||Y  
 
! BamHI ||Y||Y|| ||Y  
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! Bsu36I ||Y|| || ||
 
! Bsu36I ||Y|| || ||
 
|-
 
|-
! ClaI ||Y|| || ||
+
! ClaI ||Y|| ||Y||
 
|-
 
|-
 
! DpnI ||Y|| ||Y||   
 
! DpnI ||Y|| ||Y||   
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! FseI ||Jay's -80|| || ||  
 
! FseI ||Jay's -80|| || ||  
 
|-
 
|-
! HindIII ||Y|| ||Y||  
+
! HindIII ||Y||Y||Y||  
 
|-
 
|-
! KpnI ||Y|| ||Y||
+
! KpnI ||Y||Y|| ||Y
 
|-
 
|-
 
! MfeI || ||Y|| ||Y
 
! MfeI || ||Y|| ||Y
Line 55: Line 59:
 
! NdeI ||Y|| || ||  
 
! NdeI ||Y|| || ||  
 
|-
 
|-
! NheI || ||Y||Y||
+
! NheI ||Y||Y|| ||
 
|-
 
|-
 
! NotI ||Y||Y|| ||Y  
 
! NotI ||Y||Y|| ||Y  
 +
|-
 +
! NruI ||Y|| || ||
 
|-
 
|-
 
! PacI ||Y|| || ||
 
! PacI ||Y|| || ||
 
|-
 
|-
 
! PmeI ||Y|| || ||
 
! PmeI ||Y|| || ||
 +
|-
 +
! PstI ||Y|| || ||
 
|-
 
|-
 
! PvuII || || Y || ||
 
! PvuII || || Y || ||
Line 67: Line 75:
 
! SacI || ||Y|| ||Y
 
! SacI || ||Y|| ||Y
 
|-
 
|-
! SacII || Y || ||Y||  
+
! SacII ||Y|| ||Y||  
 
|-
 
|-
! SaII ||Y||Y|| ||Y
+
! SalI ||Y||Y|| ||Y
 
|-
 
|-
 
! SapI ||Y|| || ||
 
! SapI ||Y|| || ||
 
|-
 
|-
! SpeI ||Y|| || ||
+
! SbfI || ||Y|| ||
 +
|-
 +
! SpeI ||Y|| ||Y||
 
|-
 
|-
! SphI ||Y|| || ||
+
! SphI || ||Y|| ||
 
|-
 
|-
 
! SwaI ||Y|| || ||
 
! SwaI ||Y|| || ||

Revision as of 17:31, 15 February 2012

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Rules

  • Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common.
  • Only common enzymes may have backup, which are stored in main -20C.
  • Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI
  • If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested.
  • When the working stock has been used up, replace with a new tube.
  • Backup ligases and CIP are also in the backup cooler in main -20C.

Restriction Enzymes in Stocks

Enzyme Regular HF Regular Backup HF Backup
AflII Y
AgeI Y Y Y
AleI Y Y
ApaI Y YY
AscI Y
AsiSI Y Y
AvrII Y YY
BamHI Y Y Y
BglII Y
BsaI Y
BsrGI Y
BstBI Y
BstXI Y
Bsu36I Y
ClaI Y Y
DpnI Y Y
EcoRI Y Y
EcoRV Y Y
FseI Jay's -80
HindIII Y Y Y
KpnI Y Y Y
MfeI Y Y
NdeI Y
NheI Y Y
NotI Y Y Y
NruI Y
PacI Y
PmeI Y
PstI Y
PvuII Y
SacI Y Y
SacII Y Y
SalI Y Y Y
SapI Y
SbfI Y
SpeI Y Y
SphI Y
SwaI Y
XbaI Y Y
XhoI Y Y
XmaI Y

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