Difference between revisions of "Shreffler:Eosinophil Isolation"

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==Overview==
 
==Overview==
  
Dihydrorhodamine 123 (DHR) is a non-fluorescent, membrane permeable molecule that can loaded into cells to measure oxidative burst. Upon oxidation, it is converted to [http://en.wikipedia.org/wiki/Rhodamine rhodamine], which can be measured by flow cytometry in the fluorescein channel ([http://omlc.ogi.edu/spectra/PhotochemCAD/html/rhodamine123.html spectra]).
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Isolation of eosinophils for migration studies in collaboration with Bryan Hurley
  
 
==Materials==
 
==Materials==
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==References==
 
==References==
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*[[Special:Emailuser/Wayne G. Shreffler|Email Wayne Shreffler through OpenWetWare]]
 
*[[Special:Emailuser/Wayne G. Shreffler|Email Wayne Shreffler through OpenWetWare]]
  
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[[Category:ShreffLab]]
  
[[Catagory:Shreffler:Resources]]
 
 
[[Category:Protocol]]
 
[[Category:Protocol]]
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[[Category:Flow cytometry]]
  
 
[[Category:Immunology]]
 
[[Category:Immunology]]

Latest revision as of 09:29, 4 May 2011

Overview

Isolation of eosinophils for migration studies in collaboration with Bryan Hurley

Materials

Procedure

  1. Under hood, spray down the buffy bags and scissors with 70% EtOH and wait until dry.
  2. Cut open the buffy coat bag and pur blood into a 50 mL Falcon tube.

Discussion

discuss this protocol

References

Contact