OriC/ter ratio determination

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Revision as of 01:27, 27 February 2009 by Torsten Waldminghaus (talk | contribs) (Abstract)
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Torsten Waldminghaus

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This protocol describes how one can determine the ration of the origin of termination oriC and the terminus of replication ter in Escherichia coli. That is for example important for cell cycle analysis. To get the ratio one could use Southern Blot and quantification of the bands. However, this protocol describes the use of qPCR which is a bit faster.


List everything necessary to perform the protocol here. Include all information about suppliers, ordering details, etc. Links to the suppliers' page on that material are also appropriate and encouraged. Please be aware that users of this protocol may not be working in the same country as you.


Biological resources e.g. cell lines, buffers (link to a method for making them), enzymes, chemicals, kits, etc.


Any equipment used to perform the protocol (link to a method for using them).


A step by step guide to the experimental procedure.

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Critical steps

Referenced from the main protocol, a more thorough explanation of particularly important steps in the protocol.


Referenced from the main protocol, any comments about the protocol should be made here; i.e. how it was developed. Any comments added should be signed (by adding *'''~~~~''': in front) and explained. Links to FAQs/tips provided by other sources such as the manufacturer or other websites would be best made here.
Anecdotal observations that might be of use to others can also be posted here; e.g. 'my cells were still floating'.


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